BCH478H is designed as an advanced successor in a progression of biochemistry laboratory experiences in BCH242Y1 and BCH377H1, presenting students with a broad spectrum of techniques, approaches and concepts of contemporary biochemistry. For 2022 we are using CRISPR/Cas to construct a heterologous metabolic pathway in budding yeast. We will define genes important for pathway assembly, and test potential routes to pathway optimization. The synthetic biology will be complemented by an investigation of anti-CRISPR protein structure and function. Using state-of-the-art interaction methodologies (SPR, BLI, MST) we will analyze anti-CRISPR binding affinities. Protein crystallization, crystallization optimization, structure solving, model building, and refinement will be pursued.