Daniela Rotin

Daniela Rotin completed her PhD with Dr. Ian Tannock at the Ontario Cancer Institute, Toronto, followed by two Postdoctoral Fellowships, first at the Hospital for Sick Children, Toronto, with Dr. Sergio Grinstein, working on ion transport, and then with Dr. Joseph Schlessinger in NYU Medical Center in NY, working on Signal Transduction. She has been an Independent Investigator at the Cell Biology Program, the Hospital for Sick Children, Toronto, since 1992, where she is now a Senior Scientist, as well as a Professor at the Biochemistry Department, University of Toronto.

Biochemistry and function of the Nedd4 family of ubiquitin ligases

My laboratory has been studying the ubiquitin system, particularly the Nedd4 family of E3 ubiquitin ligases.   We are studying the biochemistry, structure and function of these E3 ligases, as well as their physiological functions, using cells and model organisms. Moreover, we have developed proteomic methodologies to globally identify substrates for E3 ubiquitin ligases and have subsequently focused our studies on some of the membrane proteins that were identified in these screens (eg the sodium channel ENaC, the FGF receptor, and several others). We also carried out interactome screens (miniTurbo/BioID) in cells and are analyzing some of the substrates identified.  More recently, we have been studying pathogenic variants (mutations) in Nedd4 family members associated with human diseases, such as hypertension, Inflammatory Bowel Disease, cancer, and others, to decipher how such mutations may cause or contribute to these diseases. 

Appointments, Cross Affiliations, Memberships

Senior Scientist Cell Biology Program, Hospital for Sick Children

Courses Taught

BCH 2110H Eukaryotic Signaling
BCH 2024H Eukaryotic Signaling
BCH426H (BCH1426H) Regulation of Signalling Pathways

Awards and Distinctions

2023 Fellow of the Royal Society of Canada
2004-2011 & 2011-2018 — Canada Research Chair, Tier I  
2011 — Woman of Action (WOA) Scientist Award (from the Israel Cancer Research Fund, ICRF)  
2003 — Zeller’s Senior Scientist Award (from the Canadian CF Foundation)  
1999-2004 — CIHR Investigator  
1999 — Premier’s Research Excellence Award (PREA)  
1994 — Power of Dreams Research Award (from the Canadian Cf Foundation)  
1993-1998 — MRC Scholarship (CIHR New Investigator)  
1993 — Elsie Winifred Crann Award (from the University of Toronto)  
1988-1991 — MRC Fellowship  
1983-1987 — NCIC Studentship Award