Circular Dichroism Spectroscopy.
The Centre for Advanced Protein Science allows scientists from various disciplines to use, apply, and develop a full range of state-of-the-art biophysical technologies to obtain a molecular description of the folding, structure, dynamics and function of protein molecules.
Some instruments are suited for the characterization of protein structure and stability such as the circular dichroism spectropolarimeter and the differential scanning calorimetry, while others are suited for the study of protein-protein and protein-ligand interactions such as the Biacore and the isothermal titration calorimeter. The instruments are available for use by the members of the general Toronto scientific community.
Equipment
- Circular dichroism spectropolarimeter – for protein structure studies
- Spectrofluorometer – for protein structure and protein-ligand interaction studies
- UV/VIS Spectrophotometer – for protein structure and protein-ligand interaction studies
- Analytical ultracentrifuge
- MALDI-ToF mass spectrometer – to determine molecular mass of proteins and peptides
- Peptide arrayer – to generate many peptides and array them on a membrane
- Isothermal titration calorimetry – for protein-ligand and protein-protein interaction studies
- Differential scanning calorimetry – to study protein stability
- Biacore – for protein-ligand and protein-protein interaction studies
- Plate Reader – to determine enzyme kinetics
Beckman Optima XL-A Analytical Ultracentrifuge
Analytical Ultracentrifugation Service
- For determining protein molecular weight and oligomerization state
- Click for information and request forms.
Locations
661 University Avenue, MaRS Centre, West Tower, 16th Floor
Funding
Instruments obtained through funds from CIHR, CFI, and Department of Biochemistry.
Coordinator and Contact
Walid A. Houry
661 University Avenue, MaRS Centre
West Tower, Room 1612
416-946-7141
walid.houry@utoronto.ca