Rubinstein lab found one of the missing puzzle pieces in the structure of the vacuolar type ATPase
Vacuolar-type ATPases (V-ATPases) are ATP-powered proton pumps involved in numerous essential processes in the cell. The Rubinstein laboratory has recently used cryo-EM to determine 3D structures of the V-ATPase (Zhao et al. 2015, Nature) and the related mitochondrial ATP synthase (Zhou et al. 2015, eLife). These cryo-EM studies have also demonstrated remarkable conformational heterogeneity in the enzymes. While conformational heterogeneity is biologically interesting, it has limited the ability to get atomic resolution structures […]