Ned van Eps

Structure and Dynamics of Complexes of G Proteins and their Receptors

16 March 2018|

Ned Van Eps in the Ernst laboratory used DEER spectroscopy together with molecular modeling techniques to map the structure of a complex between a G-protein-coupled receptor (GPCR) and its G protein. A paper describing the methodology used for characterizing the binding interface of the rhodopsin–G protein complex has recently been published in PNAS. The authors discovered that the binding mode of G protein subtypes is distinct and key differences suggest […]

How GPCRs use phosphorylation codes for arrestin recruitment

23 August 2017|

DEER spectroscopy by Ned van Eps in the Ernst lab was used to validate the crystal structure of the phosphorylated G-protein-coupled receptor (GPCR) rhodopsin in complex with arrestin. EPR measurements confirmed the location of the C-terminal tail of rhodopsin on a arrestin binding surface in a non-crystallographic environment. GPCRs are among the most important cell surface receptors controlling nearly all of our physiology. Termination of G-protein-mediated signalling by an […]

News & Views "Structural biology: Arresting developments in receptor signalling” by Jeffrey Benovic.

Ernst lab contributes DEER spectroscopy to Nature paper on rhodopsin-arrestin complex structure

6 August 2015|

The GPCR-arrestin complex crystal structure was solved using data collected at the Stanford LCLS X-ray free electron laser. Ned van Eps and Lydia Caro from the Ernst lab used pulsed electron paramagnetic resonance spectroscopy to validate the rhodopsin–arrestin complex assembly. The study published online 22 July 2015 in Nature has been downloaded more than 22,000 times in the first week after publication.