Biochemistry, University of Toronto

Mapping the molecular chaperone network

4 October 2017|

Rizzolo et al. from the Houry group provided a comprehensive view of molecular chaperone function in the cell through the use of a systematic global integrative network approach based on physical (protein-protein) and genetic (gene-gene or epistatic) interaction mapping. The analysis revealed the presence of a large chaperone functional supercomplex, which was named the NAJ chaperone complex, encompassing Hsp40, Hsp70, Hsp90, CCT and small Hsps. Many chaperones were found […]

Stagljar lab reveals new interactions among GPCRs

16 March 2017|

Using the modified membrane yeast two‐hybrid (MYTH) technology, researchers from the Stagljar lab mapped membrane protein interactions for clinically important G protein coupled receptors (GPCRs).

Read the full story on Faculty of Medicine website.

Read their paper on Molecular Systems Biology.

Stagljar lab map interactions between receptor tyrosine kinases (RTKs) and protein tyrosine phosphatases (PTPs)

1 February 2017|

Featured on the front page of Molecular Cell, the research led by Dr. Igor Stagljar captured and mapped interactions between receptor tyrosine kinases (RTKs) and protein tyrosine phosphatases (PTPs) in human cells, leading to new and improved cancer therapies.

Read the full story on the Faculty of Medicine and The Varsity websites.

Igor Stagljar and Julie Petschnigg lauded at U of T Celebrates Innovation event

15 May 2015|

At the U of T Celebrates Innovation event on May 7, the University honoured researchers and students who created technologies and successful start-up companies that have the potential for global impact and commercial appeal.

Among the 2015 Inventors of the Year are Igor Stagljar and Julie Petschnigg, who invented Mammalian membrane two-hybrid (MaMTH), which tracks membrane proteins interactions with other proteins […]

Stagljar lab uses MYTH technology to identify interactors of Sho1p

10 March 2015|

The high osmolarity glycerol (HOG) mitogen-activated protein kinase (MAPK) stress response pathway in Saccharomyces cerevisiae enables cells to adapt and remain viable under conditions of high osmotic stress; in pathogenic fungi, the HOG pathway has been implicated in virulence. HOG also serves as a model for other MAPK pathways, including those found in mammals, which are known to play a role in diseases such as Alzheimer’s.

In work recently published in […]

Igor Stagljar Ceremony at the Croatian Academy of Sciences and Arts

Igor Stagljar has been named a corresponding member of the Croatian Academy of Sciences and Arts

30 June 2014|

Professor Igor Stagljar, Donnelly Centre for Cellular & Biomolecular Research, has been named a corresponding member of the Croatian Academy of Sciences and Arts. He uses a combination of molecular, cellular, chemical genomic and proteomic approaches to study the function of yeast and human membrane proteins, as well as bacterial proteins involved in pathogenicity.

Stagljar Lab identifies a novel biomarker for cancer detection

23 March 2014|

The March 23rd issue of Nature Methods highlights the Stagljar Lab‘s development of the mammalian membrane two-hybrid (MaMTH) assay and its application to the human epidermal growth receptor (EGFR), mutations of which are associated with lung cancer. Using this approach, they identified CrkII as an interactor of the mutated EGFR and showed that Crk II regulates the stability of mutated EGFR and thus promotes signaling within […]

Dr. Igor Stagljar