The Use of High Content Screening in Biomolecular Medicine

BCH 2122H

The course will be taught at Sunnybrook Research Institute over three days and includes an additional assignment. Students will learn how to design cell-based screen assays using fluorescent cell markers/gene targets, perform a screen and acquire data using a robotic-enabled high content microscope. Material to be covered includes the philosophy and design of high content screens and the use of automated image analysis algorithms to analyze high content data sets.

Enrolled students will need to download the following and have it working on their own computer before class begins

https://cellprofiler.org/releases

and instructions on use available on that site in a popdown menu also linked here: https://cellprofiler.org/getting-started

 

Drop date: Students are not permitted to drop a 0.25 credit course if more than one class has been completed without approval of the course coordinator.

Course Next Offered

Fall 2024

Course Time and Location

Classes held: Sunnybrook Hospital, M Wing, 7th Floor

Class #1. 1 hour for SRI screening lab tour and "data drop”  to student laptops - monday, sept 16th,  1-2 pm

Class #2. 2 hr david and rob lectures class - monday, sept 23rd, 1-3 pm

Class #3. 1.5 hr for preliminary presentation and feedback - monday, october 28th 4th, 1-2:30 pm

Class #4. 2 hr for final presentation (depending on enrolment) - monday, december 2nd, 1-3 pm

Enrollment Limit

Yes — 8

Method of Student Evaluation

Participation 50%
Final data presentation 50%

Coordinator

Robert Screaton

Robert Screaton

Sunnybrook Research Institute
2075 Bayview Avenue, Room M7 617


416-480-6100 X 5743
robert.screaton@sri.utoronto.ca

David W. Andrews

David W. Andrews

Sunnybrook Health Sciences Centre
2075 Bayview Ave., Room M7 621
416-480-6100, ext. 5120
david.andrews@sri.utoronto.ca

Last Updated 21 August 2024