MYTH Schematic
The high osmolarity glycerol (HOG) mitogen-activated protein kinase (MAPK) stress response pathway in Saccharomyces cerevisiae enables cells to adapt and remain viable under conditions of high osmotic stress; in pathogenic fungi, the HOG pathway has been implicated in virulence. HOG also serves as a model for other MAPK pathways, including those found in mammals, which are known to play a role in diseases such as Alzheimer’s.
In work recently published in the Journal of Molecular Biology, the Stagljar lab used their powerful Membrane Yeast Two-Hybrid (MYTH) technology to identify interactors of the HOG pathway integral membrane protein, Sho1p. Of particular interest amongst the newly-identified interactors of Sho1p was the glycerol channel Fsp1p. In addition to previously described functions of Fsp1p, the Stagljar lab was able to show for the first time that Fsp1p is a positive regulator of Sho1p function. The work resulted in the largest confirmed interactome for Sho1p to date, has helped to delineate the HOG signaling cascade, and has identified new avenues of research in this important pathway.
