Igor Stagljar Laboratory
Department of Biochemistry
Department of Molecular Genetics
University of Toronto

Research at the Laboratory

Our lab uses a combination of molecular, cellular, chemical genomic
and proteomic approaches to study the function of yeast and
human membrane proteins, as well as bacterial proteins
involved in pathogenicity.

Current projects within the laboratory focus on four critical areas:

  1. Protein networks regulating membrane
    transport and cell signaling




  2. Protein interaction
    methodologies




  3. Chemical genomics in yeast



  4. DNA helicaces involved in
    maintenance of genome
    stability

 

Specific studies include high-throughput protein-protein interaction analyses of the yeast ABC transporters, human receptor tyrosine kinases and G-protein coupled receptors (GPCRs) using a membrane yeast two-hybrid assay developed in our lab. In addition, we have recently established a novel yeast-based assay to identify specific inhibitors of several key proteins from the opportunistic human pathogen Pseudomonas aeruginosa, and are now extending this approach for use with Plasmodium falciparum, a protozoan parasite that causes malaria in humans.

Dr. Igor Stagljar

Igor Stagljar was born in Zagreb, Croatia and educated at the University of Zagreb where he obtained a BSc degree in Molecular Biology. He then moved to Switzerland where he received his PhD degree in Molecular Biology from the Swiss Federal School of Technology (ETH) in Zurich. Afterwards he undertook two post-doctoral positions at the University of Zurich, the first working on RNA polymerase II transcription in mammals, and the second position involved working on various aspects of DNA replication and repair in yeast.

In 2001, Igor was a visiting scientist at the University of Washington in Seattle, USA, where he worked in the lab of Dr. Stanley Fields, the inventor of the yeast two-hybrid technology. From 2002 to 2005, Igor maintained an Assistant Professor position at the University of Zurich.

In the summer of 2005, Igor moved to Canada where he accepted an Associate Professor position (with tenure) at the Department of Biochemistry and Department of Molecular Genetics at the University of Toronto. In 2010, Igor was promoted to the rank of full Professor at the University of Toronto. His lab is located in the Donnelly Centre, a cutting-edge, interdisciplinary research centre in the heart of Toronto's research district.

Igor served as member or scientific officer of many peer review committees and scientific advisory boards for the national and international funding agencies as well as pharmaceutical and biotechnology companies. He is a member of the Editorial board of BioTechniques, Molecular Genetics and Genomics, BMC Biotechnology and Molecular BioSystems.

In addition, Igor is co-founder of Dualsystems Biotech, one of the world-leading interactive proteomics companies whose focus is on protein-protein interaction services, including various yeast-based two-hybrid screenings and mammalian cell-based protein complex identifications.

Lab Group Photo

Stagljar Lab group photo 2010

July 2012