Drosophila embryos stained to show the distribution of nanos mRNA (top panel) and Nanos protein (bottom panel). While nanos mRNA is concentrated at the posterior (right-hand side) of the embryo, substantial levels can be found distributed throughout the embryo. In contrast, Nanos protein specifically accumulates at the posterior of the embryo through a mechanism that ensures that only localized nanos mRNA is translated. Image courtesy of Craig Smibert.

The proper functioning of a cell and its ultimate developmental fate is determined by specific patterns of gene expression. These patterns are in part directed by signaling molecules that modulate a variety of regulatory cascades.

Gene expression begins in the nucleus, when regions of DNA are transcribed into RNA molecules, which are then processed, packaged into RNA-protein complexes, exported to the cytoplasm, transported to their ultimate destination, translated into proteins and then degraded. In addition it appears that eukaryotic transcription is inherently noisy, and it remains unclear how the relevant information is extracted from the genome.

Our research is devoted to understanding the molecular intricacies that are involved in interpretation of intercellular and intracellular regulatory signals and dictating a cell-type specific pattern of gene expression. Our interests include elucidation of the mechanisms that modulate chromatin assembly and structure; understanding the role of DNA-binding transcription factors in controlling differential transcription of a gene; determining how RNA transcripts are processed and packaged into RNA-protein complexes; understanding various quality control mechanisms that differentiate transcripts from transcriptional noise; investigating how mRNAs are transported, localized, translated and degraded.

Specific research programs in the Biochemistry Department utilize a range of model systems, from specialized mammalian cells in culture to fly embryos and employ biochemical, cell biological, computational and genome-wide approaches.

 

Faculty in the Department conducting research in this area:

Attisano Lab

Morphogen signalling pathways and the regulation of complex biological responses

Attisano Lab

160 College Street, room 1008

Dr. Liliana Attisano


416-946-3129   liliana.attisano@utoronto.ca

Bazett-Jones Lab

Ultrastructural Signature of Chromatin Domains within the Nuclei

Bazett-Jones Lab

Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay St.

Dr. David P. Bazett-Jones


416-813-2181   dbjones@sickkids.ca

Skeletal muscle regenerative medicine

Gilbert Laboratory

Terrence Donnelly Centre for Cellular and Biomolecular Research
160 College Street, Rm. # 510

Dr. Penney Gilbert


416-978-2501   penney.gilbert@utoronto.ca

Kapus Lab

The cytoskeleton as a cell fate-determining device

Kapus Lab

Keenan Centre for Biomedical Research
209 Victoria Street, Room 621

Dr. Andras Kapus


416-847-1751   KapusA@smh.ca

Ohh Lab

Molecular Mechanisms of Cancer

Ohh Lab

MaRS Centre, West Tower
661 University Avenue, Suite 1512

Dr. Michael Ohh


416-946-7922   michael.ohh@utoronto.ca

Palazzo Lab

Nuclear Export and Localization of mRNA

Palazzo Lab

MaRS, West Tower, Suite 1500
661 University Ave.

Dr. Alexander F. Palazzo


416-978-7234   alex.palazzo@utoronto.ca

Privé Lab

Protein Structure and Function

Privé Lab

Room 4-302 PMCRT
101 College Street

Dr. Gil Privé


416-581-7541   prive@uhnres.utoronto.ca

Smibert lab

Control of gene expression in the cytoplasm

Smibert lab

Medical Sciences Building, Rm 5344
1 King's College Circle

Dr. Craig A. Smibert


416-946-5538   c.smibert@utoronto.ca

Zamble Lab

Metallobiochemistry

Zamble Lab

LM443
80 St. George St.

Dr. Deborah B. Zamble


416-978-3568   deborah.zamble@utoronto.ca