Julie D. Forman-Kay

Julie D. Forman-Kay

Professor

BSc, Massachusetts Institute of Technology, 1985
PhD, Yale University, 1990
Postdoc, National Institutes of Health, 1992

Address Molecular Structure and Function
Hospital for Sick Children
Peter Gilgan Centre for Research and Learning (PGCRL)
Room 20-9713
686 Bay Street
Toronto, ON M5G 0A4
Lab Forman-Kay lab
Lab Phone 416-813-5933
Office Phone 416-813-5358
Email forman@sickkids.ca

Julie Forman-Kay is a Senior Scientist in the Molecular Structure and Function Program at the Research Institute of The Hospital for Sick Children and a Professor in the Biochemistry Department at the University of Toronto. The major focus of her work has been to provide biological insights into how dynamic properties of proteins are related to function and methodological tools to enable better understanding of dynamic and disordered states. Her expertise is in using NMR and other biophysical and computational tools to study dynamic and disordered proteins and their interactions, including characterizing their dynamic complexes that are mediated by multivalent interactions. Approaches for calculating computational representations disordered state ensembles have been developed in her group. A related area of interest is the role of post-translational modification, such as phosphorylation and methylation, in regulating structural and binding properties in disordered states and dynamic complexes.

The Forman-Kay lab works on a number of projects of specific relevance to cancer and neurobiology. Most recently her lab has started to probe the role phase separation of disordered proteins in RNA processing bodies, a key regulatory process for neurological function. Her group also has a strong interest in CFTR, the cystic fibrosis transmembrane conductance regulator, particularly its cytoplasmic domains including the disordered regulatory R region. Her work is highly collaborative and has had significant impact, with widely recognized contributions to the fields of intrinsically disordered proteins, protein interaction domains, and CFTR structure, dynamics and interactions. She is co-chair of the CFTR Structure Consortium of the US Cystic Fibrosis Foundation Therapeutics. She is the recipient of the 2012 CSMB Jeanne Manery Fisher Memorial Lectureship and the 2013 Zellers Senior Scientist Award from Cystic Fibrosis Canada.

In the News

Research Lab

Current Lab Members

Alaji Bah, Research Associate
P. Andrew Chong, Senior Research Associate
Jennifer Dawson, Research Associate
Rhea Hudson, Senior Research Associate
Hong Lin, Laboratory Research Project Coordinator
Mickael Krzeminski, Post-doctoral Fellow
Robert Vernon, Post-doctoral Fellow
Shuo-Chih Yen, MSc. Graduate Student

Collaborations

(not the complete list)
Lewis Kay, Hue Sun Chan, Mike Salter, Christine Bear, Simon Sharpe, Avi Chakrabartty, Mike Tyers, Linda Penn, Jeff Toretsky, Nahum Sonenberg, Maria Sunnerhagen, Hanoch Senderowitz, Christie Brouillette, John Hunt, Patrick Thibodeau, Robert Ford, Phil Thomas, Jack Riordan, Larry DeLucas

Major tools

NMR, fluorescence, ITC, computational work including disordered state ensemble calculations, bioinformatics and modelling

Learn more: Forman-Kay lab

Research Description

Structural Studies of Disordered Proteins

Disordered Proteins

Structural studies of intrinsically disordered proteins and regions, which play critical biological roles, lag far behind studies of folded proteins. A major focus of my work has been to bridge this gap. We developed the ENSEMBLE program to generate ensembles of conformers representing a disordered state [Marsh & Forman-Kay, Ensemble modeling of protein disordered states: Experimental restraint contributions and validation, Proteins (2012); Krzeminski et al, Characterization of disordered proteins with ENSEMBLE. Bioinformatics (2013)]. Our contributions to the field of disordered states are described in a recent review [Forman-Kay & Mittag, From sequence and forces to structure, function and evolution of intrinsically disordered proteins, 20th anniversary issue of Structure (2013)].

Dynamic Complexes

Disordered proteins often bind targets in highly dynamic complexes. We demonstrated that the disordered Sic1 cyclin dependent kinase inhibitor binds to the Cdc4 component of an SCF ubiquitin ligase complex in a dynamic complex with multiple phosphorylation sites of Sic1 exchanging on and off of the Cdc4 binding site [Mittag et al, Dynamic equilibrium engagement of a polyvalent ligand with a single site receptor, PNAS (2008); Tang et al, Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase, PNAS (2012)] and have calculated models of the free Sic1 and its dynamic complex with Cdc4, shedding insight into the ultrasensitive ubiquitination of Sic1 within the SCF ligase [Mittag et al, Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase, Structure (2010)]. We characterized Abp SH3 domain complexes involved in actin organization and correlated the degree of engagement within the dynamic complexes with functional data [Stollar et al. Structural, functional and bioinformatic studies demonstrate the crucial role of an extended peptide binding site for the SH3 domain of yeast Abp1p. J Biol Chem (2009); Stollar et al, Differential dynamic engagement within 24 SH3 domain:peptide complexes revealed by co-linear chemical shift perturbation analysis, PLoS One (2012)]. We demonstrated a dynamic interaction of 4E-BP2 that is key for translational regulation [Lukhele et al, Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface, Structure (2103)].

Importantly, we showed that phosphorylation induces folding of 40 residues of 4E-BP2 to a 4E-binding-incompatible state [Bah et al, Phosphorylation-Induced Folding in an Intrinsically Disordered Protein as a Regulatory Switch, Nature (2014)], the first time significant folding of an IDP due to post-translational modification has been reported. We have also characterized the phase separation of the disordered region of Ddx4, helping to develop this new field that links biophysics and cell biology [Nott et al, Phase transition of a disordered Nuage protein generates environmentally responsive membraneless organelles, Mol Cell (2014)].

Our studies of dynamic complexes have been highlighted in reviews [Mittag et al, Protein dynamics and conformational disorder in molecular recognition, J Mol Recognit (2010); Marsh et al, Probing the diverse landscape of protein flexibility and binding, Curr Opin Struct Biol (2012)].

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

Our studies of the dynamic interactions of the CFTR disordered R region led to a model for phospho-regulation of its activity and processing [Bozoky et al, Regulatory R region of the CFTR choloride channel is a dynamic integrator of phospho-dependent intra- and intermolecular interactions, PNAS (2013)]. Studies of the NBD1 with its dynamic regulatory elements provide insights into the molecular basis of CF [Kanelis et al, NMR evidence for differential phosphorylation-dependent interactions of wild type and ΔF508 CFTR, EMBO J (2010); Dawson et al, Allosteric coupling between the ICL4 and regulatory sites of the NBD1 of CFTR, PLoS One (2013)] and the mechanism of action of CF modulator compounds [Hudson et al, Conformational changes relevant to channel activity and folding within the first nucleotide binding domain of the cystic fibrosis transmembrane conductance regulator, J Biol Chem (2012)]. Our contributions to understanding CFTR structure and dynamics are highlighted in reviews [Chong et al, Dynamics intrinsic to cystic fibrosis transmembrane conductance regulator function and stability, Cold Spring Harb Perspect Med (2013); Bozoky et al, Structural changes of CFTR R region upon phosphorylation: a plastic platform for intramolecular and intermolecular interactions, FEBS J (2013)].

Awards & Distinctions

2016-2023 — Canada Research Chair in Intrinsically Disordered Proteins (Tier 1) Canadian Government
2013-2014 — Zellers Senior Scientist Award recognizing the outstanding contributions of an established Cystic-Fibrosis Canada-funded investigator
2012 — CSMB Jeanne Manery Fisher Memorial Lectureship
2000-2005 — Canadian Institutes of Health Research Scientist Award
1999-2004 — Ontario Government Premier’s Research Excellence Award (PREA)
1994-1999 — Medical Research Council of Canada Scholarship Award

Courses Taught

BCH374Y Research Project in Biochemistry
JBB2026H Protein Structure, Folding and Design
BCH473Y Advanced Research Project in Biochemistry

Publications

View all publications on PubMed

Coupling of tandem Smad ubiquitination regulatory factor (Smurf) WW domains modulates target specificity
P.A. Chong, H. Lin, J.L. Wrana and J.D. Forman-Kay
PNAS 107(43):18404-9 (2010)

Ensemble modeling of protein disordered states: experimental restraint contributions and validation
J.A. Marsh and J.D. Forman-Kay
Proteins 80(2):556-572 (2012)

Conformational Changes Relevant to Channel Activity and Folding within the NBD1 of CFTR
R.P. Hudson, P.A. Chong, I. Protassevitch, R. Vernon, H. Bihler, M. Mense, E. Noy, H. Senderowitz, P.J. Thomas, C. Brouillette and J.D. Forman-Kay
Journal of Biological Chemistry 287(34):28480-28494 (2012)

Differential Dynamic Engagement within 24 SH3 Domain: Peptide Complexes Revealed by Co-Linear Chemical Shift Perturbation Analysis
E.J. Stollar, H. Lin, A.R. Davidson, J.D. Forman-Kay
PLOS ONE, 7(12):e51282 (2012)

Characterization of disordered proteins with ENSEMBLE
M. Krzeminski and J.D. Forman-Kay
Bioinformatics, 29(3):398-399 (2013)

Allosteric Coupling between the Intracellular Coupling Helix 4 and Regulatory Sites of the First Nucleotide-binding Domain of CFTR
J.E. Dawson, P. Farber and J.D. Forman-Kay
PLoS One, 8(9):e74347 (2013)

Regulatory R region of the CFTR chloride channel is a dynamic integrator of phospho-dependent intra- and intermolecular interactions
Z. Bozoky, M. Krzeminski, R. Muhandiram, J. Birtley, A. Al-Zahrani, P.J. Thomas, R.A. Frizzell, R.C. Ford and J.D. Forman-Kay
Proc Natl Acad Sci USA, 110(47):E4427-36 (2013)

Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface
S. Lukhele, A. Bah, H. Lin, N. Sonenberg, J.D. Forman-Kay
Structure, 3;21(12):2186-96 (2013)

Phase transition of a disordered Nuage protein generates environmentally responsive membraneless organelles
T.J. Nott, E. Petsalakis, P. Farber, D. Jervis, E. Fussner, T. Craggs, A. Plochoweitz, D. Bazett-Jones, J.D. Forman-Kay, A.J. Baldwin, T. Pawson
Mol Cell, in press (2014)

Folding in an Intrinsically Disordered Protein by Phosphorylation as a Regulatory Switch
A. Bah, R. Vernon, Z. Siddiqui, M. Krzeminski, R. Muhandiram, C. Zhao, N. Sonenberg, L.E. Kay, J.D. Forman-Kay
Nature, in press (2014)