Christopher M. Yip

Christopher M. Yip

Professor

B.A.Sc, University of Toronto, 1988
PhD, University of Minnesota, 1996

Address 404 - 160 College St
Toronto, ON M5S 3E1
Lab Yip Lab
Lab Phone 416-946-5022
Office Phone 416-978-7853
Email christopher.yip@utoronto.ca

Professor Yip joined the University of Toronto in 1997 after completing his PhD in Chemical Engineering at the University of Minnesota in 1996. He spent one year as a post-doctoral fellow at Eli Lilly and Company in Indianapolis. He received his undergraduate degree in Chemical Engineering from the University of Toronto in 1988 and worked for Dupont Canada for 3 years before starting his graduate work at Minnesota.

Professor Christopher Yip is the Director of the University of Toronto’s Institute of Biomaterials and Biomedical Engineering, a tri-Faculty Institute.

Professor Yip currently serves on the Institute Advisory Board for the Institute of Genetics at CIHR, was the past Section Co-Chair of the NSERC Evaluation Group for Materials and Chemical Engineering, and is a standing member on the NIH Biophysics of Neural Systems Study Section.

Professor Yip enjoys endurance events, including multi-day mountain bike stage races, and trail running. He has completed 18 marathons including New York (twice) and Boston (three times) and has a marathon PB of 3:07, and a half-marathon PB of 1:28.

In the News

Research Lab

Research at the Yip Lab is quite diverse and are focused on the development and application of super-resolution combinatorial microscopies for functional cellular and molecular imaging of molecular assemblies, structures, and dynamics. Recent projects include studies of peptide and protein-membrane interactions in the context of neurodegenerative diseases and the design of novel antimicrobial agents, membrane receptor self-association and its implications for cell signaling in the context of both infection and cancer, as well as multi-colour super-resolution and light sheet microscopy.

Our lab is exceptionally well equipped for single molecular imaging and characterization

Optical microscopy

Our optical microscopes are all from Olympus and comprise the following:

  • Two confocal microscopes (Fluoview 300; Fluoview 500)
    • Systems equipped with 405 / 445 / 488 / 532 / 647 nm laser lines
  • Three total internal reflection fluorescence microscopes: two objective-based integrated with our confocal microscopes; one prism-based system
    • Systems variously equipped with 405 / 442 / 473 / 488 / 532 nm laser lines
  • Two 4-laser super-resolution microscopes
  • Several IX-70 inverted research microscopes, all equipped with epi-fluorescence capabilities
  • BX-60 upright research microscope
  • SZX-12 stereo microscope

Scanning probe microscopy

Fluorescence / Absorption spectroscopy

FT-IR spectroscopy

  • ThermoScientific /Nicolet Nexus 6700  FT-IR spectroscopy system (main bench (MCT-A detector) with mid-IR source / KBr beamsplitter; AEM module with DTGS detector; Continuum microscope with MCT-A detector equipped for reflectance / transmission / ATR-IR collection)
  • Smart Orbit ATR accessory w/ single bounce diamond / Si / Ge crystals
  • Smart Sega grazing angle reflectance accessory
  • Smart Diffuse Reflectance accessory
  • Performer Foundation Series accessory
  • ThermoScientific Nexus 670 FT-IR spectroscopy system (main bench w/ mid-IR source, MCT-A detector)
  • nanoIR platform – to be installed in Fall 2014

Optical Coherence Tomography

to be installed in Fall 2014

Learn more: Yip Lab

Research Description

Fundamental Mechanisms of Molecular Self-Assembly

Understanding and ultimately controlling how molecules assemble into functional structures is critical in fields ranging from materials science and chemistry to structural and cell biology. We have a long-standing interest in understanding the fundamental mechanisms of self-assembly, ranging from the self-association of dye molecules into excitonic structures, to protein and peptide aggregation, both in solution and at model interfaces. Our most recent work has focused on the dynamics of molecular assembly in live cells and specifically how the hetero- and homo-geneous association of membrane receptors impacts cell signaling and cell-cell interactions.

Determining the structural and conformational requirements for these complex assembly processes, and the dynamics thereof, is best accomplished by examining these phenomena on the molecular scale, in real-time, and under nominally real-world conditions. We have therefore focused on the design and optimization of combinatorial microscopies for the direct interrogation of molecular and biomolecular self-assembly. This has resulted in several platforms with unique capabilities for assessing the role of orientation, conformation, and structure on self-assembly over several orders of magnitude in both length and time.

These platforms have allowed us to

  1. identify the critical dimensions associated with exciton generation in two-dimensional molecular solids
  2. assess how protein and peptide sequence alter the dynamics of membrane association and disruption
  3. determine the spatial, conformational, and dynamic heterogeneity of membrane receptors in live cells
  4. interrogate in situ complex formation during mitochondrial membrane remodeling.

Studies of BIomolecular Self-Assembly

Toxins may exert their influence through pore complexes or other mechanisms of membrane disruption. Antimicrobial (AMP) or cell-penetrating peptides (CPP) necessarily need to pass through the membrane in order to exert their action. Intrinsic membrane proteins may require specific intermolecular interactions with specific lipids or other membrane components or structures in order to exert their function. Understanding how proteins orient, associate, and interact at a membrane interface is fundamental to understanding their function, disease progression, and devising therapeutic strategies.

The following questions need to be addressed in order to understand, control, and exploit protein-membrane interactions:

  1. What controls the association of a protein to a membrane?
  2. What changes take place upon association? Is there a change in molecular shape or orientation? How do such changes impact functionality? What is the effect on the membrane itself? Does membrane restructuring occur?
  3. How quickly does this happen?
  4. How many molecules are involved and are other molecules recruited?
  5. Where do these interactions occur? Are they dependent on membrane structure and composition and if so, what are the critical components?

Membranes can also act as templates or substrates for assembly. We are investigating the role of membrane chemistry, local structure and phase, and composition on the templated growth of novel dye aggregates. By rationally controlling the substrate structure and / or the dye chemistry, new supramolecular architectures may be generated that give rise to novel optical properties.

Single Molecule Imaging Tools and Techniques

While conventional techniques such as spectroscopy and crystallography have provided remarkable insights into biomolecular structure-function relationships, recent developments in in situ single molecule functional imaging tools are providing researchers with the unique opportunity to perform correlated characterization of protein interactions, often in real-time, and under physiologically relevant conditions, on molecular length scales.

Adapting these tools for imaging live cells, and providing direct insights into trafficking, signaling, and characterization of cellular dynamics, on single molecule length scales will provide critical new insights into the molecular mechanisms of disease and protein-protein interaction networks.

Building on the theme of combinatorial microscopies, which describes techniques that combine two or more characteristics of light (wavelength, polarization, frequency) with other imaging modalities, we are developing a platform of single molecule hyperspectral imaging tools that will enable us directly characterize protein structure and assembly, enable in situ measurement of protein association forces, and directly determine the kinetics of complex formation in live cells.

Computational Biophysics

Complementing our experimental approaches are computational techniques that can offer atomistic insights into protein-protein interactions and molecular conformations. Using all-atom molecular dynamics simulations, we are

  • characterizing the forces associated with protein unfolding, in direct analogy to single molecule force spectroscopy experiments;
  • studying peptide-membrane interactions, which is important for understanding how toxins and antimicrobial peptides interact with cell membranes;

By using these approaches in both predictive and retrospective approaches, we can assess, in silico, the role of peptide chemistry, membrane composition, and unfolding rates and forces on the dynamics of protein-protein interactions.

Awards & Distinctions

2000-2010 — Tier II Canada Research Chair
2014 — Fellow - Engineering Institute of Canada
2008 — Fellow - American Association for the Advancement of Science
2008 — University of Toronto Faculty of Medicine Graduate Faculty Teaching Award for Sustained Contribution to Excellence in Graduate Teaching
2000 — University of Toronto Faculty of Engineering and Applied Science Early Career Faculty Teaching Award
1999 — Premier's Research Excellence Award

Courses Taught

BCH425H (JBB1425) Structural Biology: Principles and Practice

Extra-Departmental Courses

BME358S Molecular Biophysics
JTC1349S Molecular Assemblies

Publications

View all publications on PubMed