September Newsletter 2021

29 September 2021|0 Comments

Click here to read the full edition: Email newsletter – Sept 2021

  • Intein

New Method to Detect Protein-Protein Interactions Developed by the Stagljar Lab

28 May 2020|0 Comments

In a new publication that was published in Nature Communications, the Stagljar lab describe a new technique, called Split Intein-Mediated Protein Ligation (SIMPL), where two test proteins are fused to two halves of intein, a protein that promotes “protein splicing”. When the two test proteins interact, the intein protein activates a splicing reaction, resulting in […]

  • Mammalian V-ATPase

First high resolution structure of a mammalian V-ATPase by the Rubinstein Lab

1 April 2020|0 Comments

In the latest issue of Science, Professor John Rubinstein’s lab reports the first high-resolution structure of a mammalian V-ATPase. These ATP-hydrolysis–driven proton pumps are essential for acidification of endosomes, lysosomes, and the trans Golgi network, as well as for acid secretion by osteoclasts, kidney intercalated cells, and some tumor cells.

ATP hydrolysis in the V-ATPase catalytic […]

  • Adrews2

Using image-based learning to localize proteins

10 March 2020|0 Comments

In the latest issue of the Journal of Cell Biology, the Andrews lab acquired >1.2 million confocal micrographs of EGFP fusion proteins localized at key cell organelles in murine and human cells from which morphology and statistical features were measured. Machine learning algorithms they developed that use these features to permit automated assignment of the […]

  • MaMTH-DS

New potential anti-cancer compounds discovered by the Stagljar lab

1 March 2020|0 Comments

In the latest issue of Nature Chemical Biology, the Stagljar lab used the mammalian membrane two-hybrid drug screening (MaMTH-DS), a technique that they previously pioneered, to identify new compounds that inhibit the activity of oncoproteins that cause breast cancer. In this case they found four compounds that inhibit the dimerization of the epidermal growth factor […]