• Intein

New Method to Detect Protein-Protein Interactions Developed by the Stagljar Lab

28 May 2020|0 Comments

In a new publication that was published in Nature Communications, the Stagljar lab describe a new technique, called Split Intein-Mediated Protein Ligation (SIMPL), where two test proteins are fused to two halves of intein, a protein that promotes “protein splicing”. When the two test proteins interact, the intein protein activates a splicing reaction, resulting in […]

  • Mammalian V-ATPase

First high resolution structure of a mammalian V-ATPase by the Rubinstein Lab

1 April 2020|0 Comments

In the latest issue of Science, Professor John Rubinstein’s lab reports the first high-resolution structure of a mammalian V-ATPase. These ATP-hydrolysis–driven proton pumps are essential for acidification of endosomes, lysosomes, and the trans Golgi network, as well as for acid secretion by osteoclasts, kidney intercalated cells, and some tumor cells.

ATP hydrolysis in the V-ATPase catalytic […]

  • MaMTH-DS

New potential anti-cancer compounds discovered by the Stagljar lab

1 March 2020|0 Comments

In the latest issue of Nature Chemical Biology, the Stagljar lab used the mammalian membrane two-hybrid drug screening (MaMTH-DS), a technique that they previously pioneered, to identify new compounds that inhibit the activity of oncoproteins that cause breast cancer. In this case they found four compounds that inhibit the dimerization of the epidermal growth factor […]

  • NOD2

How bacterial invaders are sensed in the gut

25 October 2019|0 Comments

A new paper in the latest issue of Science describes how the bacterial-sensing proteins, NOD1/2, are recruited to the membranes of our gut cells for them to function to initiate a proper immune response. This involves the addition of a lipid, palmitoyl, to NOD1/2 by the palmitoyl-transferase ZDHHC5. Importantly variants of NOD2 that are associated […]

  • BandC

Red Cell Anion Exchanger Makes the Cover of Biophysical Journal

1 October 2019|0 Comments

Dario De Vecchis, a post-doctoral fellow in the laboratory of Dr. Antreas Kalli at Leeds, University, in a collaborative project with Reinhart Reithmeier used molecular dynamics simulations to build the first atomic scale model of the red cell anion exchanger in a complex lipid bilayer that mimics the native membrane. This work appeared in the […]