Laurence A. Moran Professor

B.Sc., Carleton University, 1968
Ph.D., Princeton University, 1974
University of Geneva, 1974-78

Medical Sciences Building, Room 5253
416-978-2704
l.moran@utoronto.ca
Blog: Sandwalk

Molecular Evolution



Research Synopsis
 
I am interested in molecular evolution and evolutionary theory. As a model system I have been working with a family of genes known as the HSP70 multigene family. One of the members of this gene family is the major heat shock inducible gene that is expressed in response to stress in all organisms. Other family members are transcribed normally in almost all cells. In addition there are special members of the multigene family that are expressed only in the cells of certain tissues or at specific times of development.

The HSP70 gene family is attractive because these genes are so important to life on this planet. These are the most highly conserved genes known in all of biology and their protein products act as molecular chaperones that assist in the proper folding of proteins and the assembly and repair of macromolecular structures inside of the cell.

In the 1980's my lab cloned and sequenced several members of the HSP70 multigene family from Drosophila, mouse and yeast cells. We have combined these sequences with hundreds of other HSP70 sequences to create an HSP70 Sequence Database. By aligning these sequences we are able to construct phylogenetic trees that reveal the deepest relationships of all living species.




            


Textbooks
Moran, L.A., Horton, R. Scrimgeour, K. G., and Perry, M.D. Principles of Biochemistry 5th. ed. Pearson/Prentice Hall (2012)

Horton, R., Moran, L.A., Scrimgeour, K. G., Perry, M.D., and Rawn, J.D. Principles of Biochemistry 4th. ed. Pearson/Prentice Hall (2006)

Horton, R., Moran, L.A., Ochs, R., Rawn, J.D. and Scrimgeour, K. G. Principles of Biochemistry 3rd. ed. Prentice Hall (2001)

Horton, R., Moran, L.A., Ochs, R., Rawn, J.D. and Scrimgeour, K. G. Principles of Biochemistry 2nd. ed. Prentice Hall (1996)

Moran, L.A. and Scrimgeour, K.G. Moran/Scrimgeour Biochemistry Resource Book Neil Patterson Publishers/Prentice Hall (1994)

Moran, L.A., Scrimgeour, K.G., Horton, H.R., Ochs, R.S. and Rawn, J.D. Biochemistry Neil Patterson Publishers/Prentice Hall (1994)

Horton, R., Moran, L.A., Ochs, R., Rawn, J.D. and Scrimgeour, G. Principles of Biochemistry Neil Patterson Publishers (Simon & Shuster) (1993)

Rawn, J.D. Biochemistry Neil Patterson Publishers (1989) (Chapters 23-29)



Internet Publications
The HSP70 Sequence Database Moran, L.A. (1994-2006).

The HSP70 Aligned Sequence Database Moran, L.A. (1994-2006).

Evolution by Accident Moran, L.A. (2006).

Random Genetic Drift Moran, L.A. (2006).

What Is Evolution? Moran, L.A.(2006).

Theistic Evolution: The Fallacy of the Middle Ground Moran, L.A.(2006).

The Modern Synthesis of Genetics and Evolution Moran, L.A. (2005).

Evolution Is a Fact and a Theory Moran, L.A. (2005).

Human HSP70 Genes Uygungil, B. and Moran, L.A. (2004, 2006).

BCH299Y: Molecular Evolution Moran, L.A. (2006).

Random Genetic Drift Moran, L.A. (1994, 1997).

The Modern Synthesis of Genetics and Evolution Moran, L.A. (1994, 1997).

Evolution Is a Fact and a Theory Moran, L.A. (1994, 1997).

What Is Evolution? Moran, L.A. (1994, 1997).



Selected Publications
Structure and expression of an inducible HSP70-encoding gene from Mus musculus. Perry, M.D., Aujame, L., Shtang, S., and Moran, L.A. (1994) Gene 146, 273-278.

An essential member of the HSP70 gene family of S. cerevisiae is homologous to immunoglobulin heavy chain binding protein R.C. Nicholson, Williams, D.B. and Moran, L.A. (1990) Proc. Natl. Acad. Sci. 86, 1159-1163.

Inducible expression of an hsp68-lacZ hybrid gene in transgenic mice Kothary, R., Clapoff, S., Darling, S., Perry, M., Moran, L.A. and Rossant, J. (1989) Development 105, 707-714.

Isolation of a mouse heat-shock gene (hsp68) by recombinational screening. Perry, M.D. & Moran, L.A. (1987) Gene 51, 227-236.

Cell-lineage-specific expression of the mouse hsp68 gene during embryogenesis. Kothary, R., Perry, M.D., Moran, L.A. & Rossant. J. (1987) Devel. Biol. 121, 342-348.

Molecular cloning and analysis of DNA complementary to three mouse Mr = 68,000 heat shock protein mRNAs. Lowe, D.G. & Moran, L.A. (1986) J. Biol. Chem. 261, 2102-2112.

Expression of heat shock genes in fetal and maternal rabbit brain. Brown, I.R., Lowe, D.G. & Moran, L.A. (1985) Neurochem. Res. 10, 1277-1284.

Expression of a Drosophila heat-shock gene in cells of the yeast Saccharomyces cerevisiae. Nicholson, R.C. & Moran, L.A. (1984) Biosci. Rep. 4, 963-972.
   

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