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Laurence A. Moran
Professor
B.Sc., Carleton University, 1968
Ph.D., Princeton University, 1974
University of Geneva, 1974-78 |
Medical Sciences
Building, Room 5253
416-978-2704
l.moran@utoronto.ca
Blog: Sandwalk |
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Molecular Evolution
Research Synopsis
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I am interested in molecular evolution and evolutionary theory.
As a model system I have been working with a family
of genes known as the HSP70 multigene family. One of the
members of this gene family is the major heat shock inducible
gene that is expressed in response to stress in all organisms.
Other family members are transcribed normally in almost
all cells. In addition there are special members of the
multigene family that are expressed only in the cells
of certain tissues or at specific times of development.
The HSP70 gene family is attractive because these genes are so
important to life on this planet. These are the most highly
conserved genes known in all of biology and their protein products
act as molecular chaperones that assist in the proper folding
of proteins and the assembly and repair of macromolecular
structures inside of the cell.
In the 1980's my lab cloned and sequenced several members of the
HSP70 multigene family from Drosophila, mouse and yeast
cells. We have combined these sequences with hundreds of other HSP70
sequences to create an HSP70 Sequence
Database. By aligning these sequences we are able to construct phylogenetic trees that
reveal the deepest relationships of all living species.
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Textbooks
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Moran, L.A., Horton, R. Scrimgeour, K. G., and Perry, M.D. Principles of
Biochemistry 5th. ed. Pearson/Prentice Hall (2012)
Horton, R., Moran, L.A., Scrimgeour, K. G., Perry, M.D., and Rawn, J.D. Principles of
Biochemistry 4th. ed. Pearson/Prentice Hall (2006)
Horton, R., Moran, L.A., Ochs, R., Rawn, J.D. and Scrimgeour, K. G. Principles of
Biochemistry 3rd. ed. Prentice Hall (2001)
Horton, R., Moran, L.A., Ochs, R., Rawn, J.D. and Scrimgeour, K. G. Principles of
Biochemistry 2nd. ed. Prentice Hall (1996)
Moran, L.A. and Scrimgeour, K.G. Moran/Scrimgeour Biochemistry Resource Book Neil
Patterson Publishers/Prentice Hall (1994)
Moran, L.A., Scrimgeour, K.G., Horton, H.R., Ochs, R.S. and Rawn, J.D.
Biochemistry Neil
Patterson Publishers/Prentice Hall (1994)
Horton, R., Moran, L.A., Ochs, R., Rawn, J.D. and Scrimgeour, G. Principles of
Biochemistry Neil Patterson Publishers (Simon & Shuster) (1993)
Rawn, J.D. Biochemistry Neil Patterson Publishers (1989) (Chapters 23-29)
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Internet Publications
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The HSP70 Sequence Database Moran, L.A.
(1994-2006).
The
HSP70 Aligned Sequence Database Moran, L.A.
(1994-2006).
Evolution
by Accident Moran, L.A. (2006).
Random
Genetic Drift Moran, L.A. (2006).
What Is
Evolution? Moran, L.A.(2006).
Theistic
Evolution: The Fallacy of the Middle Ground Moran, L.A.(2006).
The
Modern Synthesis of Genetics and Evolution Moran, L.A. (2005).
Evolution
Is a Fact and a Theory Moran, L.A. (2005).
Human HSP70
Genes Uygungil, B. and Moran, L.A. (2004, 2006).
BCH299Y: Molecular Evolution Moran,
L.A. (2006).
Random Genetic
Drift Moran,
L.A. (1994, 1997).
The Modern Synthesis of
Genetics and
Evolution Moran, L.A. (1994, 1997).
Evolution Is a Fact and a
Theory Moran, L.A. (1994, 1997).
What Is
Evolution? Moran, L.A. (1994, 1997).
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Selected Publications
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Structure and expression of an inducible HSP70-encoding gene from Mus
musculus. Perry, M.D., Aujame, L., Shtang, S., and Moran, L.A. (1994) Gene 146, 273-278.
An essential
member of the HSP70 gene family of S. cerevisiae is homologous
to immunoglobulin heavy chain binding protein R.C. Nicholson,
Williams, D.B. and Moran, L.A. (1990) Proc. Natl. Acad.
Sci. 86, 1159-1163.
Inducible expression of an hsp68-lacZ hybrid gene in transgenic
mice Kothary, R., Clapoff, S., Darling, S., Perry, M.,
Moran, L.A. and Rossant, J. (1989) Development 105, 707-714.
Isolation of a mouse heat-shock gene (hsp68) by recombinational
screening. Perry, M.D. & Moran, L.A. (1987) Gene 51, 227-236.
Cell-lineage-specific expression of the mouse hsp68 gene
during embryogenesis. Kothary, R., Perry, M.D., Moran,
L.A. & Rossant. J. (1987) Devel. Biol. 121, 342-348.
Molecular cloning and analysis of DNA complementary to
three mouse Mr = 68,000 heat shock protein mRNAs. Lowe,
D.G. & Moran, L.A. (1986) J. Biol. Chem. 261, 2102-2112.
Expression of heat shock genes in fetal and maternal rabbit
brain. Brown, I.R., Lowe, D.G. & Moran, L.A. (1985) Neurochem.
Res. 10, 1277-1284.
Expression of a Drosophila heat-shock gene in cells of
the yeast Saccharomyces cerevisiae. Nicholson, R.C. &
Moran, L.A. (1984) Biosci. Rep. 4, 963-972. |
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