Kelley Laboratory - Cellular and Molecular Sensors

ResearchPeoplePublicationsPositions AvailableShana O. Kelley
DR. SHANA O. KELLEY

PROFESSOR

Faculty of Pharmacy, Faculty of Medicine, Biochemistry

phone: 416-978-8641

email:

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EDUCATION
1999
Ph.D.
California Institute of Technology
1994
B.S.
Seton Hall University


PROFESSIONAL APPOINTMENTS
2008
Director, Division of Biomolecular Sciences, Faculty of Pharmacy
University of Toronto
2006
Professor
University of Toronto
2006
Professor
Boston College
2000-2006
Assistant Professor
Boston College
1999-2000
NIH Postdoctoral Fellow
Scripps Research Institute


AWARDS AND HONOURS
2008
Named one of "Canada's Top 40 under 40" by Globe & Mail/Caldwell Partners
2006
Pittsburgh Conference Achievement Award
2005
Camille Dreyfus Teacher-Scholar Award
2005
Boston College Distinguished Research Award
2004
TR100: Voted one of the top 100 innovators by Technology Review Magazine
2004
Alfred P. Sloan Fellowship
2004
NSF CAREER Award
2002
Boston College Research Incentive Award
2000
Dreyfus New Faculty Award
2000
Research Innovation Award


PUBLICATIONS

KELLEY GROUP RESEARCH

Biosensing and bionanomaterials

  1. "Programming the Detection Limits of Biosensors through Controlled Nanostructuring."  L. Soleymani, Z. Fang, E.H. Sargent, S.O. Kelley, Nature Nanotechnology, 2009, in press, DOI: 10.1038/nnano.2009.276.

  2. "Direct Profiling of Cancer Biomarkers in Tumour Tissue Using a Multiplexed Nanostructured Microelectrode Integrated Circuit."  H. Yang, A. Hui, G. Pampalakis, L. Soleymani, F.-F. Liu, E. H. Sargent, S.O. Kelley, ACS Nano, 2009, in press, DOI: 10.1021/nn900733d.

    3. The papers appearing in ACS Nano and Nature Nanotechnology received extensive media coverage and were featured by the National Post, Scientific American, and CBC National News/Mansbrisge One on One.

  3. "Direct, Electronic MicroRNA Detection Reveals Differential Expression Profiles in 30 Minutes."  H. Yang, A. Hui, G. Pampalakis, L. Soleymani, F.-F. Liu, E. H. Sargent, S.O. Kelley, Angewandte Chemie, 2009, 48, 8461.

  4. "Nanostructuring of Patterned Microelectrodes to Enhance the Sensitivity of Electrochemical Nucleic Acids Detection."  L. Soleymani, X. Sun, H. Yang, B.J. Taft, E. H. Sargent, S.O. Kelley, Angewandte Chemie, 2009, 48, 8457.

  5. "One-step Synthesis of DNA-Programmed Semiconductor Nanocrystals."  N. Ma, E.H. Sargent, S.O. Kelley, Nature Nanotechnology, 2009, 4, 121.

  6. "Direct Electrocatalytic mRNA Detection Using PNA-Nanowire Sensors."  Z. Fang, S.O. Kelley, Anal. Chem., 2009, 81, 612.

  7. "An Electrochemical Immunosensor Based on Antibody-Nanowire Conjugtes."  G. Pampalakis, S.O. Kelley, The Analyst, 2009, 134, 447

  8. "A Comparison of Quality of Dispersion of Single Wall Carbon Nanotubes using Different Surfactants and Biomolecules."  R. Haggenmueller, S. Rahatekar, J. Fagan, J. Chun, M. Becker, T. Krauss, R. Naik, L. Carlson, J, Kadla, P. Trulove, D. Fox, Z. Fang, S.O.Kelley, J. Gilman*, Langmuir, 2008, 24, 5070.

  9. "DNA-Passivated CdS Nanocrystals: Luminescence Bioimaging and Toxicity Profiles."  N. Ma, J. Yang, K.M. Stewart, S.O. Kelley*, Langmuir, 2007, 23, 12783.
  10. "Nucleotide-Stabilized Cadmium Sulfide Nanoparticles".  C. J. Dooley, J. Rouge, N. Ma, M. Invernale, S. O. Kelley*, J. Mat. Chem, 2007, 17, 1687.
  11. "NIR-emitting Colloidal Quantum Dots having 26% Luminescence Quantum Yield in Buffer Solution."  S. Hinds, S. Myrskog, L. Levina, G. Koleilat, J. Yang, S.O. Kelley*, E.H. Sargent*, J. Am. Chem. Soc., 2007, 129, 7218.
  12. "Ultrasensitive Detection of Enzymatic Activity with Nanowire Electrodes." M.A. Roberts, S.O. Kelley*, J. Am. Chem. Soc., 2007, 129, 11356.
  13. "RNA-Templated Semiconductor Nanocrystals" N. Ma, C.J. Dooley, and S.O. Kelley*, J. Am. Chem.Soc., 2006, 128, 12598. (featured in Nature Nanotechnology).
  14. "Heterogeneous Deposition of Noble Metals on Semiconductor Nanocrystals" J. Yang, L. Levina, E.H. Sargent, S.O. Kelley*, J. Mater. Chem., 2006, 16, 4025.
  15. "Microfluidic Three-Electrode Cell Array for Low-Current Electrochemical Detection" N. Trirog, M.A. Lapierre-Devlin, S.O. Kelley, and J.R. Beresford*, IEEE Sensors, 2006, 128, 1395.
  16. "Opto-electrical Characteristics of ZnO Nanorods Grown by DNA-Directed Assembly on a Carbon Nanotube Array" A.D. Lazareck, S.G. Cloutier, T.-F. Kuo, B.J. Taft, S.O. Kelley, and J.M. Xu*, Appl. Phys. Lett., 2006, 89, 103109.
  17. "DNA-Directed Synthesis of ZnO Nanowires on Carbon Nanotube Tips" A.D. Lazareck, S.G. Cloutier, T.F. Kuo, B. J. Taft, S.O. Kelley, and J.M. Xu*, Nanotechnology, 2006, 17, 2661.
  18. "An Intercalator Film as a DNA-Electrode Interface." B.J. Taft, M.A. Lapierre-Devlin, B.J. Taft, S.O. Kelley*, Chem. Comm., 2006, 9, 962.
  19. "Programming Nanoparticle Growth using Nucleic Acid Ligands" S. Hinds, B.J. Taft, L. Levina, V. Sukhovatkin, M.D. Roy, C.J. Dooley, M.D. Roy, E.H. Sargent*, S.O. Kelley*, J. Am. Chem. Soc., 2006, 128, 64.
  20. "Amplified Electrocatalysis at DNA-Modified Nanowires." M.A. Lapierre-Devlin, C. Asher, B.J. Taft, R. Gasparac, M.W. Roberts, S.O. Kelley*, Nano Letters, 2005, 5, 1051.
  21. "Site-Specific Delivery of DNA and Appended Cargo to Carbon Nanotubes." B.J. Taft, A.D. Lazareck, G.D. Withey, A. Yin, J.M. Xu, S.O. Kelley*, J. Am. Chem. Soc., 2004, 126, 12750.
  22. "Ultrasensitive DNA Detection at 2D and 3D Nanoelectrodes." R. Gasparac, B.J. Taft, M. Lapierre-Devlin, A.D. Lazareck, J.M. Xu, S.O. Kelley*, J. Am. Chem. Soc., 2004, 126, 12270.
  23. "Electrocatalytic Detection of Pathogenic DNA Sequences and Antibiotic Resistance Markers." M.A. Lapierre, M.M. O'Keefe, B.J. Taft, S.O. Kelley*, Analytical Chemistry, 2003, 75, 6327.
  24. "Engineering DNA-Electrode Linkages: Manipulation of Linker Length and Electronics." B.J. Taft, M.M. O'Keefe, J.T. Fourkas, S.O. Kelley*, Analytica Chimica Acta, 2003, 496, 81-91 (invited).

Peptide conjugates as cellular and molecular probes

  1. "Mitochondria-Penetrating Peptides: Sequence Effects and Model Cargo Transport." L. Yousif, K.M. Stewart, K.L. Horton, S.O. Kelley, ChemBioChem, 2009, 10, 2081.

  2. "Engineered Apoptosis-Inducing Peptides with Enhanced Mitochondrial Localization and Potency." K.L. Horton, S.O. Kelley, J. Med. Chem., 2009, 52, 3293.

  3. "Mitochondria-Penetrating Peptides."  K.L. Horton, K.M. Stewart, S.B. Fonseca, Q. Guo, S.O. Kelley, Chemistry & Biology, 2008, 15, 375. (featured by the Faculty of 1000, Nature Methods and C&E News)

  4. "Cyanine Dye Conjugates as Probes for Live Cell Imaging." J.R. Carreon, K.M. Stewart, K.P. Mahon, S. Shin, S.O. Kelley, Bioorg. Med. Chem. Lett., 2007, 17, 5182.

  5. "Deconvolution of the Oxidative Stress Response using Organelle-Specific Peptides." K.P. Mahon, T.B. Potocky, D. Blair, M.D. Roy, T.C. Chiles, S.O. Kelley, Chemistry and Biology, 2007, 14, 923. (Cover article)

  6. "Tunable DNA Photocleavage by Peptidointercalator Conjugates" K.P. Mahon, M.D. Roy, J.R. Carreon, E.G. Prestwich, J. Rouge, S. Shin and S.O. Kelley*, Chem. Bio. Chem., 2006, 7, 766.
  7. "Oxidative DNA Strand Scission Induced by Peptides.” E.G. Prestwich, M.D. Roy, J. Rego, and S.O. Kelley, Chemistry and Biology, 2005, 12, 695.
  8. "Phototoxicity of Peptidoconjugates Modulated by a Single Amino Acid." L.M. Wittenhagen, J.R. Carreon, E.G. Prestwich, S.O. Kelley*, Angewandte Chemie, 2005, 44, 2542.
  9. "Synthesis, Characterization, and Cellular Uptake of DNA-Binding Rose Bengal Peptidoconjugates." J.R. Carreon, M.A. Roberts, L.M. Wittenhagen, S.O. Kelley*, Organic Letters, 2005, 7, 99.
  10. "Thiazole Orange-Peptide Conjugates: Sensitivity of DNA Binding to Chemical Structure." J.R. Carreon, K.P. Mahon, S.O. Kelley*, Organic Letters, 2004, 6, 517.
  11. "Photosensitized DNA Cleavage Promoted by Amino Acids." K.P. Mahon, R.F. Ortiz Meoz, E.G. Prestwich, S.O. Kelley*, Chemical Communications, 2003, 15, 1956. (featured in C+E News, 7/21/03)

Disease-related tRNAs

  1. "A Single Residue in Leucyl-tRNA Synthetase Affecting Amino Acid Specificity and tRNA Aminoacylation." S.W. Lue and S.O. Kelley, Biochemistry, 2007, 46, 4466.

  2. "A Defunct Editing Site in a Human Aminoacyl-tRNA Synthetase." S.W. Lue, and S.O. Kelley*, Biochemistry, 2005, 44, 3010.
  3. "Solution Structure of a Pathogenic tRNA Dimer." M.D. Roy, L.M. Wittenhagen, S.O. Kelley*, RNA, 2005, 11, 254.
  4. "Combinatorial Analysis of tRNA Substrates for hs mt LeuRS." E. Zagriadska, S.O. Kelley*, Biochemistry, 2005, 44, 233.
  5. "Interdomain Communication in a Disease-Related Human tRNA." M.D. Roy, L.M. Wittenhagen, and S.O. Kelley*, Biochemistry, 2004, 43, 384.
  6. "The Pathogenic U3271C Human Mitochondrial tRNALeu Mutation Disrupts a Fragile Anticodon Stem." L.M. Wittenhagen, M.D. Roy, and S.O. Kelley*, Nucleic Acids Research, 2003, 2, 596.
  7. "Dimerization of a Pathogenic Human tRNA." L.M. Wittenhagen and S.O. Kelley*, Nature Structural Biology, 2002, 9, 586.

KELLEY GROUP RESEARCH REVIEWS AND OTHER PUBLICATIONS

  1. "Recent Advances in the Biological and Medical Applications of Cell-Penetrating Peptides." S.B. Fonseca, M. Pereira, S.O. Kelley, Adv. Drug. Del. Rev. 2009, 61, 953.

  2. "Targeting Mitochondria with Organelle-Specific Compounds: Strategies and Applications." L. Yousif, K.M. Stewart, and S.O. Kelley, ChemBioChem, 2009, 10, 1939.
  4. "Nanomaterials for Ultrasensitive Electrochemical Biosensing." H. Lord, S.O. Kelley, J. Matl. Chem., 2009, 19, 3127.
  6. "Cell-Permeable Peptides as Delivery Vehicles for Biology and Medicine." K.M. Stewart, K.L. Horton, S.O. Kelley, Org. Biomol. Chem., 2008, 6, 2242.

  7. "Biotemplated Nanostructures:  Directed Assembly of Electronic and Optical Materials using Nanoscale Complementarity."  N. Ma, E.H.Sargent, S.O. Kelley, J. Mat. Chem., 2008, 18, 954 .

  8. "Electrochemistry at the DNA/Electrode Interface: New Approaches to Nucleic Acids Biosensing." S.O. Kelley and M.G. Hill, Bioinorganic Electrochemistry, Springer, Netherlands, 2008.
  9. "Structural Perturbations in Disease-Related tRNAs." S.O. Kelley*, L.M. Wittenhagen, M.D. Roy, Sequence-Dependent Curvature and Deformation in Nucleic Acids and Protein-Nucleic Acid Complexes (ACS Symposium Series 844), 2004, 177-188.
  10. "Impact of Disease-Related Mitochondrial Mutations on tRNA Structure and Function." L.M. Wittenhagen, S.O. Kelley*, Trends in Biochemical Sciences, 2003, 28, 605-611.
  11. "DNA Pioneer Helped Open Doors for Women." S.O. Kelley, Boston Herald, 2/23/03 (op-ed).
  12. "Charge Migration Through Double Helical DNA." S.O. Kelley*, Electroanalytical Methods for Biological Materials, Marcel Dekker, NY, 2002.

S.O. KELLEY POSTDOCTORAL AND THESIS RESEARCH
  1. "Synthesis and Spectroelectrochemistry of Ir(bpy)(phen)(phi)3+." C.S. Stinner, M.D. Wightman, S.O. Kelley, M.G. Hill, J.K. Barton, Inorganic Chemistry, 2001, 40, 5245-5250.
  2. "Fragile T-stem in Disease-associated Human Mitochondrial tRNA Sensitizes Structure to Local and Distant Mutations." S.O. Kelley, S. V. Steinberg, and P. Schimmel, Journal of Biological Chemistry, 2001, 276, 10607-10611.
  3. "Luminescence Quenching by DNA-Bound Viologens: Effect of Reactant Identity on the Efficiency of Electron Transfer in DNA." S.O. Kelley, G. Orellana, and J.K. Barton, Journal of Photochemistry and Photobiology, B: Biology, 2000, 58, 72-79.
  4. "Pathogenic Functional Defects of a tRNA Related to Structural Fragility." S.O. Kelley, Sergey V. Steinberg, and P. Schimmel, Nature Structural Biology 2000, 7, 862-865.
  5. "Exiting an RNA World." P. Schimmel and S.O. Kelley, Nature Structural Biology, 2000, 7, 5-7.
  6. "Single-Base Mismatch Detection Based on Charge Transduction Through DNA." S.O. Kelley, E.M. Boon, N.M. Jackson, M.G. Hill, and J.K. Barton, Nucleic Acids Research, 1999, 27, 4830-4837.
  7. "Femtosecond Dynamics of DNA-Mediated Electron Transfer." T. Fiebig, S.O. Kelley, C. Wan, C. R. Treadway, J.K. Barton, A. Zewail, Proceedings of the National Academy of Sciences U.S.A., 1999, 96, 6014-6019.
  8. "Femtosecond Dynamics of the DNA Intercalator Ethidium and Electron Transfer with Mononucleotides in Water." T. Fiebig, C. Wan, S.O. Kelley, J.K. Barton, A. Zewail, Proceedings of the National Academy of Sciences U.S.A., 1999, 96, 1187-1192.
  9. "Long-Range Electron Transfer Through DNA Monolayers." S.O. Kelley, N.M. Jackson, M.G. Hill, and J.K. Barton, Angewandte Chemie Intl. Ed. Eng., 1999, 38, 941-945.
  10. "Electron Transfer between Bases in Double Helical DNA." S.O. Kelley and J.K. Barton, Science, 1999, 283, 375-383.
  11. "Orienting DNA Helices on Gold Using Applied Fields." S.O. Kelley, J. K. Barton, N.M. Jackson, L. MacPherson, A. Potter, E.M. Spain, M.J. Allen, and M.G. Hill, Langmuir, 1998, 14, 6781-6784.
  12. "Long-Range and Short-Range Oxidative Damage to DNA: Photoinduced Damage to Guanines in Ethidium-DNA Assemblies." D.B. Hall, S.O. Kelley, J.K. Barton, Biochemistry, 1998, 37, 15933-15940.
  13. "Radical Migration Through the DNA Helix: Chemistry at a Distance." S.O. Kelley and J.K. Barton, (invited review) Metal Ions in Biological Systems, 1998, 26, 211-249.
  14. "DNA-Mediated Electron Transfer from Ethidium to a Modified Base." S.O. Kelley and J.K. Barton, Chemistry and Biology, 1998, 8, 413-425.
  15. "Photoinduced Electron Transfer in Ethidium-Modified Duplexes." S.O. Kelley, R.E. Holmlin, E.D.A. Stemp, and J.K. Barton, Journal of the American Chemical Society, 1997, 119, 9861-9870.
  16. "Electrochemistry of Methylene Blue Bound to a DNA-Modified Electrode." S.O. Kelley, J. K. Barton, N.M. Jackson, and M.G. Hill, Bioconjugate Chemistry, 1997, 8, 31-37.