Advanced Biochemistry Lab

BCH478H

BCH478H is designed as an advanced successor in a progression of biochemistry laboratory experiences in BCH242Y1, BCH377H1, and BCH378H1, presenting students with a broad spectrum of techniques, approaches and concepts of contemporary biochemistry. For 2018 we are using CRISPR/Cas to construct a heterologous metabolic pathway in budding yeast. We will follow this with an assessment of anti-CRISPR activity in vivo. The synthetic biology will be complemented by an investigation of anti-CRISPR protein structure and function. Using state-of-the-art interaction methodologies (SPR, BLI, MST) we will analyze anti-CRISPR binding affinities. Protein crystallization, crystallization optimization, structure solving, model building, and refinement will be pursued.

Course Next Offered

September 2018

Course Time and Location

Tuesdays 3 to 5 pm AND Fridays ~10 am to ~5 pm
Teaching Labs, Medical Sciences Building, Room 2384

Prerequisites

BCH340H1, BCH371H1/(BCH377H1, BCH378H1), MGY311Y1

Enrollment Limit

None

Exclusions

Restricted to Sp. Biochemistry

Recommended Reading

The laboratory manual will be provided to students on Portal.  There is no required textbook for this course.

Frequent reference is made to original papers and review articles.

Coordinator

Grant W. Brown

Grant W. Brown

Donnelly Centre, Room 1206
160 College Street
416-946-5733
grant.brown@utoronto.ca

Trevor F. Moraes

Trevor F. Moraes

MaRS Center, West Tower
661 University Ave., Rm 1613
416-946-3048
trevor.moraes@utoronto.ca

Instructors

Last Updated 20 March 2018