This course deals with the secretory and endocytic pathways of eukaryotic cells. It examines secreted and membrane proteins as they move from their site of synthesis in the endoplasmic reticulum (ER) through to the Golgi and the plasma membrane, or are sorted to intracellular organelles. The specific topics covered include protein translocation into the ER, chaperones and protein folding in the ER, retrotranslocation and protein degradation, the Unfolded Protein Response (UPR), vesicle biogenesis and ER to Golgi transport, regulated secretion, endocytosis and protein sorting in polarized cells.