Biochemistry Laboratory I

BCH377H

This is an introductory laboratory course for specialist students in Biochemistry, Immunology, and Molecular Genetics. Experiments in this course aim to illustrate and build upon some of the concepts described in BCH242Y, and serve as a foundation for more advanced training in subsequent biochemistry laboratory courses.

The topics covered include:

  • Properties of buffers.
  • Enzyme properties and kinetics (Michaelis-Menten analyses).
  • Protein purification and analysis, including chromatography, spectrophotometric protein assays, enzyme assays, SDS-PAGE and mass spectrometry analysis.
  • Isolation of genomic DNA, PCR analysis, and agarose gel electrophoresis.
  • Methods to study protein unfolding (chemical and thermal denaturation), and protein expression, stability and degradation (western blotting and enzyme assays).

The first class will be on Tuesday, Sept. 13th, 2016 beginning at 9 am sharp! (Room MSB 4171). Visit Portal for more information.

Course Next Offered

September 2016

Course Time and Location

Tuesdays 9 am to 1 pm AND Thursdays 9 to 10 am.

Teaching Labs, Medical Sciences Building

Visit Portal for detailed schedule and room locations.

Prerequisites

BCH242Y

Enrollment Limit

Yes —

Enrollment Controls
R: restricted to students who are enrolled in:
Sp. Biochemistry
Sp. Immunology
Sp. Molecular Genetics & Microbiology (Genetics stream)
Enrolment is limited.

Exclusions

BCH370H, BCH371H, CHM379H

Method of Student Evaluation

30% Assignments
25% Journal-Style Report
15% Performance Evaluation
30% Final Exam

A more detailed description of each component will be provided in class and on Portal.

Recommended Reading

The laboratory manual will be provided on Portal for students to print out themselves.  There is no textbook required for this course.  Recommended (optional) resources are listed below.

 

Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A.J. Ninfa and D.P. Ballou, Fitzgerald Scientific Press, 1998.

Biochemical Calculations, 2nd ed. Segel, I.H. John Wiley & Sons, Inc., New York (1976)

Course Coordinator 2016 – Helen Miliotis

 

Additional Notes and Resources

Helen Miliotis (Course Coordinator for the Fall 2016 Academic Session) MSB 5221A, helen.miliotis@utoronto.ca

Last Updated 31 August 2016