Researchers in Prosser Lab and Pai Lab recently published in Science on the Role of Dimer Asymmetry and Protomer Dynamics in Enzyme Catalysis.
An interaction between the respiratory enzyme fumarate reductase (Frd, also known as Complex II) and an ATPase has been identified in E. coli by the Houry lab. The research shows that the RavA ATPase, belonging to a poorly characterized but ubiquitous MoxR family of AAA+ ATPases, associates with the Frd respiratory enzyme through an adaptor, which the group named ViaA. […]
The laboratory of Dr. Oliver Ernst has used X-ray crystallography to determine the structure of a membrane protein that never left a lipid-bilayer environment (i.e., without the use of conventional detergents). The work, published in Structure and highlighted on the Journal’s cover, was led by postdoctoral fellow Dr. Jana Broecker. Polymer-bounded lipid nanodiscs were used to extract and purify membrane proteins with their surrounding lipids and allowed […]
Many bacterial pathogens modulate their hosts through complex arsenals of effector proteins that are injected into host cell during infection. Indeed, the concept that effectors target host proteins and processes to modulate their activities is central to the current molecular understanding of host-pathogen interactions. Legionella pneumophila, the causative agent of a deadly pneumonia known as Legionnaires’ disease, has over 300 effectors, which is the largest known arsenal amongst bacterial pathogens. […]
Newly made proteins which do not quite achieve the correct 3D shape in the ER are moved to the cell cytoplasm via a specific membrane pore, and broken down. Many disease causing gene mutations e.g. in cystic fibrosis, also result in misfolding of the mutant protein, and its transport though this pore, for cytoplasmic degradation. This pore is hijacked by some bacterial toxins which need to access the cytoplasm to […]
The dimeric enzyme fluoroacetate dehalogenase is one of only a handful of protein catalysts that can break the strongest bond in organic chemistry, the one between carbon and fluorine atoms, in the process transforming the highly toxic pesticide fluoroacetate into glycolate, a benign molecule. The laboratories of Emil Pai and Scott Prosser, together with the group of Régis Pomès and collaborators in the US and Japan, have […]
Stagljar lab map interactions between receptor tyrosine kinases (RTKs) and protein tyrosine phosphatases (PTPs)
Featured on the front page of Molecular Cell, the research led by Dr. Igor Stagljar captured and mapped interactions between receptor tyrosine kinases (RTKs) and protein tyrosine phosphatases (PTPs) in human cells, leading to new and improved cancer therapies.
In work led by postdoctoral fellow Mohammad T. Mazhab-Jafari, the Rubinstein laboratory used electron cryomicroscopy (cryo-EM) to determine the atomic structure of the membrane-bound region of a eukaryotic V-ATPase. This study, which was published in Nature, gives the first high-resolution structure for the membrane region of any rotary ATPase, a family of enzymes that includes proton pumping V-ATPases and proton-driven ATP synthases. The structure reveals several surprising features of […]
Natalie Bamford, a former University of Toronto Biochemistry Specialist and current PhD student in the Howell lab, was recently awarded a prestigious Vanier Canada Graduate Scholarship from the Natural Sciences and Engineering Research Council of Canada (NSERC) to continue her studies. Natalie’s research focuses on understanding the biosynthesis of the fungal biofilm exopolysaccharide galactosaminogalactan.
“This is a new exciting project as little is known of fungal exopolysaccharide biosynthesis. I […]
A group led by Peter Kim and Robert Bandsma (Paediatrics) made a breakthrough published in Journal of Cell Biology and in the Journal of Hepatology in linking peroxisome function through the gene PEX2 and the cell’s response to starvation.
Malnourished children living in the same household who are given the same food to eat sometimes have stark differences in health, leading researchers to query why some severely malnourished […]