Russell E. Bishop Assistant Professor

B.Sc., University of Alberta, Biochemistry, 1989
Ph.D., University of Alberta, Biochemistry, 1997
PostDoc, University of Alberta, PENCE, 1997
PostDoc, Duke University, Biochemistry, 1998-2000


Medical Sciences Building, Room 6213
416-946-7103
russell.bishop@utoronto.ca

Note: Dr. Bishop has moved to McMaster University
Contact him at:

http://www.fhs.mcmaster.ca/biochem/dir/fac/bishop.htm


Biogenesis of the Gram-negative Cell Envelope

Links to Training Programs:
CIHR Strategic Training Program in the Structural Biology of Membrane Proteins Linked to Disease
CIHR Strategic Training Program in Protein Folding: Principles and Diseases
Collaborative Graduate Program in Proteomics and Bioinformatics


 


Research Synopsis

Our research on the structure and function of membrane proteins attacks one of the most important and challenging problems in contemporary biochemistry. Our most recent work has been focussed on an outer membrane enzyme PagP from pathogenic Gram-negative bacteria.  PagP transfers a palmitoyl group from a phospholipid molecule to the lipid A or endotoxin component of lipopolysaccharide. This simple modification provides bacterial resistance to host antimicrobial peptides and attenuates the inflammatory response signaled through the toll-like receptor 4 pathway.  By understanding how the structural and dynamic properties of PagP relate to its biological function, we hope to design inhibitors that might be useful for the treatment of infections and to synthesize novel endotoxin antagonists for the treatment of septic shock.
 


Selected Publications

Jia W, El Zoeiby A, Petruzziello TN, Jayabalasingham B, Seyedirashti S, Bishop RE.  Lipid trafficking controls endotoxin acylation in outer membranes of Escherichia coli.  J Biol Chem. 2004 Aug 19; 279: 44966-75.

Ahn VE, Lo EI, Engel CK, Chen L, Hwang PM, Kay LE, Bishop RE, Prive GG.  A
hydrocarbon ruler measures palmitate in the enzymatic acylation of endotoxin. EMBO J. 2004 Aug 4;23(15):2931-41.

Hwang PM, Bishop RE, Kay LE.  The integral membrane enzyme PagP alternates
between two dynamically distinct states. Proc Natl Acad Sci U S A. 2004 Jun 29; 101(26):9618-23.

Bishop RE, Lo EI, Khan MA, El Zoeiby A, Jia W. Enzymology of lipid A
palmitoylation in bacterial outer membranes. J Endotoxin Res. 2004;10(2):107-12.

Kim SH, Jia W, Bishop RE, Gyles C. An msbB homologue carried in plasmid
pO157 encodes an acyltransferase involved in lipid A biosynthesis in Escherichia coli O157:H7. Infect Immun. 2004 Feb;72(2):1174-80.

Hwang PM, Choy WY, Lo EI, Chen L, Forman-Kay JD, Raetz CR, Prive GG, Bishop RE, Kay LE. Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13560-5.

Bishop RE.The bacterial lipocalins. Biochim Biophys Acta. 2000 Oct 18;1482 (1-2):73-83.

Bishop RE, Gibbons HS, Guina T, Trent MS, Miller SI, Raetz CR. Transfer of
palmitate from phospholipids to lipid A in outer membranes of gram-negative
bacteria. EMBO J. 2000 Oct 2;19(19):5071-80.


All Publications

   

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